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Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data
BACKGROUND: Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some s...
Autores principales: | Olova, Nelly, Krueger, Felix, Andrews, Simon, Oxley, David, Berrens, Rebecca V., Branco, Miguel R., Reik, Wolf |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856372/ https://www.ncbi.nlm.nih.gov/pubmed/29544553 http://dx.doi.org/10.1186/s13059-018-1408-2 |
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