Cargando…

Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR

Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms behind blood-induced PCR inhibition were evaluated by use of whole blood as well as known PCR-inhibitory molecules in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sidstedt, Maja, Hedman, Johannes, Romsos, Erica L., Waitara, Leticia, Wadsö, Lars, Steffen, Carolyn R., Vallone, Peter M., Rådström, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2018
Materias:	Paper in Forefront
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857286/ https://www.ncbi.nlm.nih.gov/pubmed/29504082 http://dx.doi.org/10.1007/s00216-018-0931-z

Ejemplares similares

PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
por: Sidstedt, Maja, et al.
Publicado: (2020)

Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
por: Pavšič, Jernej, et al.
Publicado: (2015)

Evaluation of the performance of quantitative detection of the Listeria monocytogenes prfA locus with droplet digital PCR
por: Witte, Anna Kristina, et al.
Publicado: (2016)

Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR
por: Niu, Chunyan, et al.
Publicado: (2021)

A fully automated microfluidic PCR-array system for rapid detection of multiple respiratory tract infection pathogens
por: Huang, Enqi, et al.
Publicado: (2021)

Blending DNA binding dyes to improve detection in real-time PCR
por: Jansson, Linda, et al.
Publicado: (2017)

Quantitation of methotrexate polyglutamates in human whole blood, erythrocytes and leukocytes collected via venepuncture and volumetric absorptive micro-sampling: a green LC–MS/MS-based method
por: Daraghmeh, Dala N., et al.
Publicado: (2022)

Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM
por: Leitner, Michael, et al.
Publicado: (2017)

Supporting non-target identification by adding hydrogen deuterium exchange MS/MS capabilities to MetFrag
por: Ruttkies, Christoph, et al.
Publicado: (2019)

Point-of-care human milk testing for maternal secretor status
por: Chung, Saeromi, et al.
Publicado: (2021)

Investigation of native and aggregated therapeutic proteins in human plasma with asymmetrical flow field-flow fractionation and mass spectrometry
por: Ramm, Ingrid, et al.
Publicado: (2022)

Hydrophobic ionic liquids for quantitative bacterial cell lysis with subsequent DNA quantification
por: Fuchs-Telka, Sabine, et al.
Publicado: (2016)

Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum
por: Leeman, Mats, et al.
Publicado: (2020)

Effects of leachates from UV-weathered microplastic on the microalgae Scenedesmus vacuolatus
por: Rummel, Christoph D., et al.
Publicado: (2021)

Affine analysis for quantitative PCR measurements
por: Patrone, Paul N., et al.
Publicado: (2020)

Biochemical profiling of rat embryonic stem cells grown on electrospun polyester fibers using synchrotron infrared microspectroscopy
por: Doncel-Pérez, Ernesto, et al.
Publicado: (2018)

The strength in numbers: comprehensive characterization of house dust using complementary mass spectrometric techniques
por: Rostkowski, Pawel, et al.
Publicado: (2019)

Reducing Bias and Quantifying Uncertainty in Fluorescence Produced by PCR
por: DeJaco, Robert F., et al.
Publicado: (2023)

Detailed structural elucidation of polyesters and acrylates using Fourier transform mass spectrometry
por: Simonsick, William J., et al.
Publicado: (2008)

An on-line post-column detection system for the detection of reactive-oxygen-species-producing compounds and antioxidants in mixtures
por: Kool, Jeroen, et al.
Publicado: (2007)

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS
por: Varga, Elisabeth, et al.
Publicado: (2012)

Development of a multiplex DNA-based traceability tool for crop plant materials
por: Voorhuijzen, Marleen M., et al.
Publicado: (2011)

NMR methods to monitor the enzymatic depolymerization of heparin
por: Limtiaco, John F. K., et al.
Publicado: (2010)

Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis
por: Weiss, Jana M., et al.
Publicado: (2009)

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification
por: Devonshire, Alison S., et al.
Publicado: (2014)

N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application
por: Kosman, Joanna, et al.
Publicado: (2014)

Macroscopic and microscopic spatially-resolved analysis of food contaminants and constituents using laser-ablation electrospray ionization mass spectrometry imaging
por: Nielen, Michel W. F., et al.
Publicado: (2014)

Determination of porphyrins in oral bacteria by liquid chromatography electrospray ionization tandem mass spectrometry
por: Fyrestam, Jonas, et al.
Publicado: (2015)

In vitro RNA release from a human rhinovirus monitored by means of a molecular beacon and chip electrophoresis
por: Weiss, Victor U., et al.
Publicado: (2016)

Determination of aromatic amines in human urine using comprehensive multi-dimensional gas chromatography mass spectrometry (GCxGC-qMS)
por: Lamani, Xolelwa, et al.
Publicado: (2014)

Critical evaluation of indirect methods for the determination of deoxynivalenol and its conjugated forms in cereals
por: Malachová, Alexandra, et al.
Publicado: (2015)

Development and application of a UHPLC–MS/MS metabolomics based comprehensive systemic and tissue-specific screening method for inflammatory, oxidative and nitrosative stress
por: Schoeman, Johannes C., et al.
Publicado: (2018)

Automated clean-up, separation and detection of polycyclic aromatic hydrocarbons in particulate matter extracts using a 2D-LC/2D-GC system: a method translation from two FIDs to two MS detectors
por: Lim, Hwanmi, et al.
Publicado: (2017)

Silicone wristbands compared with traditional polycyclic aromatic hydrocarbon exposure assessment methods
por: Dixon, Holly M., et al.
Publicado: (2018)

Geochemical wolframite fingerprinting – the likelihood ratio approach for laser ablation ICP-MS data
por: Martyna, Agnieszka, et al.
Publicado: (2018)

Liquid chromatographic nanofractionation with parallel mass spectrometric detection for the screening of plasmin inhibitors and (metallo)proteinases in snake venoms
por: Zietek, Barbara M., et al.
Publicado: (2018)

The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase
por: Bollella, Paolo, et al.
Publicado: (2018)

Affinity profiling of monoclonal antibody and antibody-drug-conjugate preparations by coupled liquid chromatography-surface plasmon resonance biosensing
por: Lakayan, Dina, et al.
Publicado: (2018)

Virus-like particle size and molecular weight/mass determination applying gas-phase electrophoresis (native nES GEMMA)
por: Weiss, Victor U., et al.
Publicado: (2019)

Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray
por: Nybond, Susanna, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_b5ilrsj5s8664f8mnbn84h9tll