Coupling next-generation sequencing to dominant positive screens for finding antibiotic cellular targets and resistance mechanisms in Escherichia coli

In order to expedite the discovery of genes coding for either drug targets or antibiotic resistance, we have developed a functional genomic strategy termed Plas-Seq. This technique involves coupling a multicopy suppressor library to next-generation sequencing. We generated an Escherichia coli plasmi...

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Detalles Bibliográficos
Autores principales: Gingras, Hélène, Dridi, Bédis, Leprohon, Philippe, Ouellette, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857375/
https://www.ncbi.nlm.nih.gov/pubmed/29319470
http://dx.doi.org/10.1099/mgen.0.000148

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5857375/
https://www.ncbi.nlm.nih.gov/pubmed/29319470
http://dx.doi.org/10.1099/mgen.0.000148

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