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PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation

Germ cell tumors (GCTs) are a heterogeneous group of tumors occurring in gonadal and extragonadal locations. GCTs are hypothesized to arise from primordial germ cells (PGCs), which fail to differentiate. One recently identified susceptibility loci for human GCT is PR (PRDI-BF1 and RIZ) domain protei...

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Autores principales: Gell, Joanna J., Zhao, Jasmine, Chen, Di, Hunt, Timothy J., Clark, Amander T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858915/
https://www.ncbi.nlm.nih.gov/pubmed/29324254
http://dx.doi.org/10.1016/j.scr.2017.12.016
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author Gell, Joanna J.
Zhao, Jasmine
Chen, Di
Hunt, Timothy J.
Clark, Amander T.
author_facet Gell, Joanna J.
Zhao, Jasmine
Chen, Di
Hunt, Timothy J.
Clark, Amander T.
author_sort Gell, Joanna J.
collection PubMed
description Germ cell tumors (GCTs) are a heterogeneous group of tumors occurring in gonadal and extragonadal locations. GCTs are hypothesized to arise from primordial germ cells (PGCs), which fail to differentiate. One recently identified susceptibility loci for human GCT is PR (PRDI-BF1 and RIZ) domain proteins 14 (PRDM14). PRDM14 is expressed in early primate PGCs and is repressed as PGCs differentiate. To examine PRDM14 in human GCTs we profiled human GCT cell lines and patient samples and discovered that PRDM14 is expressed in embryonal carcinoma cell lines, embryonal carcinomas, seminomas, intracranial germinomas and yolk sac tumors, but is not expressed in teratomas. To model constitutive overexpression in human PGCs, we generated PGC-like cells (PGCLCs) from human pluripotent stem cells (PSCs) and discovered that elevated expression of PRDM14 does not block early PGC formation. Instead, we show that elevated PRDM14 in PGCLCs causes proliferation and differentiation defects in the germline.
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spelling pubmed-58589152018-03-19 PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation Gell, Joanna J. Zhao, Jasmine Chen, Di Hunt, Timothy J. Clark, Amander T. Stem Cell Res Article Germ cell tumors (GCTs) are a heterogeneous group of tumors occurring in gonadal and extragonadal locations. GCTs are hypothesized to arise from primordial germ cells (PGCs), which fail to differentiate. One recently identified susceptibility loci for human GCT is PR (PRDI-BF1 and RIZ) domain proteins 14 (PRDM14). PRDM14 is expressed in early primate PGCs and is repressed as PGCs differentiate. To examine PRDM14 in human GCTs we profiled human GCT cell lines and patient samples and discovered that PRDM14 is expressed in embryonal carcinoma cell lines, embryonal carcinomas, seminomas, intracranial germinomas and yolk sac tumors, but is not expressed in teratomas. To model constitutive overexpression in human PGCs, we generated PGC-like cells (PGCLCs) from human pluripotent stem cells (PSCs) and discovered that elevated expression of PRDM14 does not block early PGC formation. Instead, we show that elevated PRDM14 in PGCLCs causes proliferation and differentiation defects in the germline. 2018-01-04 2018-03 /pmc/articles/PMC5858915/ /pubmed/29324254 http://dx.doi.org/10.1016/j.scr.2017.12.016 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Gell, Joanna J.
Zhao, Jasmine
Chen, Di
Hunt, Timothy J.
Clark, Amander T.
PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
title PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
title_full PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
title_fullStr PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
title_full_unstemmed PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
title_short PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
title_sort prdm14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858915/
https://www.ncbi.nlm.nih.gov/pubmed/29324254
http://dx.doi.org/10.1016/j.scr.2017.12.016
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