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Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy

As an image-based single-cell transcriptomics approach, multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows hundreds to thousands of RNA species to be identified, counted and localized in individual cells while preserving the native spatial context of RNAs. In MERFISH, RNAs...

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Detalles Bibliográficos
Autores principales: Wang, Guiping, Moffitt, Jeffrey R., Zhuang, Xiaowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859009/
https://www.ncbi.nlm.nih.gov/pubmed/29555914
http://dx.doi.org/10.1038/s41598-018-22297-7