Evaluation of two main RNA-seq approaches for gene quantification in clinical RNA sequencing: polyA+ selection versus rRNA depletion

To allow efficient transcript/gene detection, highly abundant ribosomal RNAs (rRNA) are generally removed from total RNA either by positive polyA+ selection or by rRNA depletion (negative selection) before sequencing. Comparisons between the two methods have been carried out by various groups, but t...

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Detalles Bibliográficos
Autores principales: Zhao, Shanrong, Zhang, Ying, Gamini, Ramya, Zhang, Baohong, von Schack, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859127/
https://www.ncbi.nlm.nih.gov/pubmed/29556074
http://dx.doi.org/10.1038/s41598-018-23226-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859127/
https://www.ncbi.nlm.nih.gov/pubmed/29556074
http://dx.doi.org/10.1038/s41598-018-23226-4

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