Affinity maturation of a portable Fab–RNA module for chaperone-assisted RNA crystallography

Antibody fragments such as Fabs possess properties that can enhance protein and RNA crystallization and therefore can facilitate macromolecular structure determination. In particular, Fab BL3–6 binds to an AAACA RNA pentaloop closed by a GC pair with ∼100 nM affinity. The Fab and hairpin have served...

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Detalles Bibliográficos
Autores principales: Koirala, Deepak, Shelke, Sandip A, Dupont, Marcel, Ruiz, Stormy, DasGupta, Saurja, Bailey, Lucas J, Benner, Steven A, Piccirilli, Joseph A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861428/
https://www.ncbi.nlm.nih.gov/pubmed/29309709
http://dx.doi.org/10.1093/nar/gkx1292

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861428/
https://www.ncbi.nlm.nih.gov/pubmed/29309709
http://dx.doi.org/10.1093/nar/gkx1292

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