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Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis

BACKGROUND: Quantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entirely depe...

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Detalles Bibliográficos
Autores principales: Nguyen, Duc Quan, Eamens, Andrew L., Grof, Christopher P. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861610/
https://www.ncbi.nlm.nih.gov/pubmed/29581727
http://dx.doi.org/10.1186/s13007-018-0293-8