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Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis

MicroScale Thermophoresis (MST) is a frequently used method for the quantitative characterization of intermolecular interactions with several advantages over other technologies. One of these is its capability to determine equilibrium constants in solution including complex biological matrices such a...

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Detalles Bibliográficos
Autores principales: Bartoschik, Tanja, Galinec, Stefanie, Kleusch, Christian, Walkiewicz, Katarzyna, Breitsprecher, Dennis, Weigert, Sebastian, Muller, Yves A., You, Changjiang, Piehler, Jacob, Vercruysse, Thomas, Daelemans, Dirk, Tschammer, Nuska
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862892/
https://www.ncbi.nlm.nih.gov/pubmed/29563556
http://dx.doi.org/10.1038/s41598-018-23154-3