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Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis
MicroScale Thermophoresis (MST) is a frequently used method for the quantitative characterization of intermolecular interactions with several advantages over other technologies. One of these is its capability to determine equilibrium constants in solution including complex biological matrices such a...
Autores principales: | Bartoschik, Tanja, Galinec, Stefanie, Kleusch, Christian, Walkiewicz, Katarzyna, Breitsprecher, Dennis, Weigert, Sebastian, Muller, Yves A., You, Changjiang, Piehler, Jacob, Vercruysse, Thomas, Daelemans, Dirk, Tschammer, Nuska |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862892/ https://www.ncbi.nlm.nih.gov/pubmed/29563556 http://dx.doi.org/10.1038/s41598-018-23154-3 |
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