Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging

CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dC...

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Detalles Bibliográficos
Autores principales: Hong, Yu, Lu, Guangqing, Duan, Jinzhi, Liu, Wenjing, Zhang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863892/
https://www.ncbi.nlm.nih.gov/pubmed/29566733
http://dx.doi.org/10.1186/s13059-018-1413-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863892/
https://www.ncbi.nlm.nih.gov/pubmed/29566733
http://dx.doi.org/10.1186/s13059-018-1413-5

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