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Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dC...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863892/ https://www.ncbi.nlm.nih.gov/pubmed/29566733 http://dx.doi.org/10.1186/s13059-018-1413-5 |