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Methodology challenges in studying human gut microbiota – effects of collection, storage, DNA extraction and next generation sequencing technologies

The information on microbiota composition in the human gastrointestinal tract predominantly originates from the analyses of human faeces by application of next generation sequencing (NGS). However, the detected composition of the faecal bacterial community can be affected by various factors includin...

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Detalles Bibliográficos
Autores principales: Panek, Marina, Čipčić Paljetak, Hana, Barešić, Anja, Perić, Mihaela, Matijašić, Mario, Lojkić, Ivana, Vranešić Bender, Darija, Krznarić, Željko, Verbanac, Donatella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865204/
https://www.ncbi.nlm.nih.gov/pubmed/29572539
http://dx.doi.org/10.1038/s41598-018-23296-4