One-step methodology for the direct covalent capture of GPCRs from complex matrices onto solid surfaces based on the bioorthogonal reaction between haloalkane dehalogenase and chloroalkanes

Protein immobilization techniques play an important role in the development of assays for disease diagnosis and drug discovery. However, many of these approaches are not applicable to transmembrane proteins. G protein-coupled receptors (GPCRs) are the largest protein superfamily encoded by the human...

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Detalles Bibliográficos
Autores principales: Zeng, Kaizhu, Li, Qian, Wang, Jing, Yin, Guowei, Zhang, Yajun, Xiao, Chaoni, Fan, Taiping, Zhao, Xinfeng, Zheng, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868316/
https://www.ncbi.nlm.nih.gov/pubmed/29629116
http://dx.doi.org/10.1039/c7sc03887a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868316/
https://www.ncbi.nlm.nih.gov/pubmed/29629116
http://dx.doi.org/10.1039/c7sc03887a

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