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Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis

BACKGROUND: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. METHODS: The potential interferences...

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Autores principales: Deng, Guoying, Dai, Chenyun, Chen, Jinyuan, Ji, Anqi, Zhao, Jingpeng, Zhai, Yue, Kang, Yingjie, Liu, Xijian, Wang, Yin, Wang, Qiugen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868597/
https://www.ncbi.nlm.nih.gov/pubmed/29606872
http://dx.doi.org/10.2147/IJN.S159776
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author Deng, Guoying
Dai, Chenyun
Chen, Jinyuan
Ji, Anqi
Zhao, Jingpeng
Zhai, Yue
Kang, Yingjie
Liu, Xijian
Wang, Yin
Wang, Qiugen
author_facet Deng, Guoying
Dai, Chenyun
Chen, Jinyuan
Ji, Anqi
Zhao, Jingpeng
Zhai, Yue
Kang, Yingjie
Liu, Xijian
Wang, Yin
Wang, Qiugen
author_sort Deng, Guoying
collection PubMed
description BACKGROUND: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. METHODS: The potential interferences Se@SiO(2) nanocomposites may have to the therapeutic effect of methylprednisolone (MPS) were evaluated by classical therapeutic effect index of acute respiratory distress syndrome (ARDS), such as wet-to-dry weight ratio, inflammatory factors IL-1β and TNF-α. And oxidative stress species (ROS) index like superoxide dismutase (SOD) and glutathione (GSH) were tested. Then, the protection effects of Se@SiO(2) have in osteonecrosis of the femoral head (ONFH) were evaluated by micro CT, histologic analysis and Western-blot analysis. RESULTS: In the present study, we found that in the rat model of ARDS, Se@SiO(2) nanocomposites induced SOD and GSH indirectly to reduce ROS damage. The wet-to-dry weight ratio of lung was significantly decreased after MPS treatment compared with the control group, whereas the Se@SiO(2) did not affect the reduced wet-to-dry weight ratio of MPS. Se@SiO(2) also did not impair the effect of MPS on the reduction of inflammatory factors IL-1β and TNF-α, and on the alleviation of structural destruction. Furthermore, micro CT and histologic analysis confirmed that Se@SiO(2) significantly alleviate MPS-induced destruction of femoral head. Moreover, compared with MPS group, Se@SiO(2) could increase collagen II and aggrecan, and reduce the IL-1β level in the cartilage of femoral head. In addition, the biosafety of Se@SiO(2) in vitro and in vivo were supported by cell proliferation assay and histologic analysis of main organs from rat models. CONCLUSION: Se@SiO(2) nanocomposites have a protective effect in MPS-induced ONFH without influence on the therapeutic activity of MPS, suggesting the potential as effective drugs to avoid ONFH in MPS therapy.
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spelling pubmed-58685972018-03-30 Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis Deng, Guoying Dai, Chenyun Chen, Jinyuan Ji, Anqi Zhao, Jingpeng Zhai, Yue Kang, Yingjie Liu, Xijian Wang, Yin Wang, Qiugen Int J Nanomedicine Original Research BACKGROUND: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. METHODS: The potential interferences Se@SiO(2) nanocomposites may have to the therapeutic effect of methylprednisolone (MPS) were evaluated by classical therapeutic effect index of acute respiratory distress syndrome (ARDS), such as wet-to-dry weight ratio, inflammatory factors IL-1β and TNF-α. And oxidative stress species (ROS) index like superoxide dismutase (SOD) and glutathione (GSH) were tested. Then, the protection effects of Se@SiO(2) have in osteonecrosis of the femoral head (ONFH) were evaluated by micro CT, histologic analysis and Western-blot analysis. RESULTS: In the present study, we found that in the rat model of ARDS, Se@SiO(2) nanocomposites induced SOD and GSH indirectly to reduce ROS damage. The wet-to-dry weight ratio of lung was significantly decreased after MPS treatment compared with the control group, whereas the Se@SiO(2) did not affect the reduced wet-to-dry weight ratio of MPS. Se@SiO(2) also did not impair the effect of MPS on the reduction of inflammatory factors IL-1β and TNF-α, and on the alleviation of structural destruction. Furthermore, micro CT and histologic analysis confirmed that Se@SiO(2) significantly alleviate MPS-induced destruction of femoral head. Moreover, compared with MPS group, Se@SiO(2) could increase collagen II and aggrecan, and reduce the IL-1β level in the cartilage of femoral head. In addition, the biosafety of Se@SiO(2) in vitro and in vivo were supported by cell proliferation assay and histologic analysis of main organs from rat models. CONCLUSION: Se@SiO(2) nanocomposites have a protective effect in MPS-induced ONFH without influence on the therapeutic activity of MPS, suggesting the potential as effective drugs to avoid ONFH in MPS therapy. Dove Medical Press 2018-03-22 /pmc/articles/PMC5868597/ /pubmed/29606872 http://dx.doi.org/10.2147/IJN.S159776 Text en © 2018 Deng et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Deng, Guoying
Dai, Chenyun
Chen, Jinyuan
Ji, Anqi
Zhao, Jingpeng
Zhai, Yue
Kang, Yingjie
Liu, Xijian
Wang, Yin
Wang, Qiugen
Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
title Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
title_full Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
title_fullStr Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
title_full_unstemmed Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
title_short Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
title_sort porous se@sio(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868597/
https://www.ncbi.nlm.nih.gov/pubmed/29606872
http://dx.doi.org/10.2147/IJN.S159776
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