Cargando…
Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis
BACKGROUND: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. METHODS: The potential interferences...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868597/ https://www.ncbi.nlm.nih.gov/pubmed/29606872 http://dx.doi.org/10.2147/IJN.S159776 |
_version_ | 1783309161973415936 |
---|---|
author | Deng, Guoying Dai, Chenyun Chen, Jinyuan Ji, Anqi Zhao, Jingpeng Zhai, Yue Kang, Yingjie Liu, Xijian Wang, Yin Wang, Qiugen |
author_facet | Deng, Guoying Dai, Chenyun Chen, Jinyuan Ji, Anqi Zhao, Jingpeng Zhai, Yue Kang, Yingjie Liu, Xijian Wang, Yin Wang, Qiugen |
author_sort | Deng, Guoying |
collection | PubMed |
description | BACKGROUND: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. METHODS: The potential interferences Se@SiO(2) nanocomposites may have to the therapeutic effect of methylprednisolone (MPS) were evaluated by classical therapeutic effect index of acute respiratory distress syndrome (ARDS), such as wet-to-dry weight ratio, inflammatory factors IL-1β and TNF-α. And oxidative stress species (ROS) index like superoxide dismutase (SOD) and glutathione (GSH) were tested. Then, the protection effects of Se@SiO(2) have in osteonecrosis of the femoral head (ONFH) were evaluated by micro CT, histologic analysis and Western-blot analysis. RESULTS: In the present study, we found that in the rat model of ARDS, Se@SiO(2) nanocomposites induced SOD and GSH indirectly to reduce ROS damage. The wet-to-dry weight ratio of lung was significantly decreased after MPS treatment compared with the control group, whereas the Se@SiO(2) did not affect the reduced wet-to-dry weight ratio of MPS. Se@SiO(2) also did not impair the effect of MPS on the reduction of inflammatory factors IL-1β and TNF-α, and on the alleviation of structural destruction. Furthermore, micro CT and histologic analysis confirmed that Se@SiO(2) significantly alleviate MPS-induced destruction of femoral head. Moreover, compared with MPS group, Se@SiO(2) could increase collagen II and aggrecan, and reduce the IL-1β level in the cartilage of femoral head. In addition, the biosafety of Se@SiO(2) in vitro and in vivo were supported by cell proliferation assay and histologic analysis of main organs from rat models. CONCLUSION: Se@SiO(2) nanocomposites have a protective effect in MPS-induced ONFH without influence on the therapeutic activity of MPS, suggesting the potential as effective drugs to avoid ONFH in MPS therapy. |
format | Online Article Text |
id | pubmed-5868597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58685972018-03-30 Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis Deng, Guoying Dai, Chenyun Chen, Jinyuan Ji, Anqi Zhao, Jingpeng Zhai, Yue Kang, Yingjie Liu, Xijian Wang, Yin Wang, Qiugen Int J Nanomedicine Original Research BACKGROUND: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. METHODS: The potential interferences Se@SiO(2) nanocomposites may have to the therapeutic effect of methylprednisolone (MPS) were evaluated by classical therapeutic effect index of acute respiratory distress syndrome (ARDS), such as wet-to-dry weight ratio, inflammatory factors IL-1β and TNF-α. And oxidative stress species (ROS) index like superoxide dismutase (SOD) and glutathione (GSH) were tested. Then, the protection effects of Se@SiO(2) have in osteonecrosis of the femoral head (ONFH) were evaluated by micro CT, histologic analysis and Western-blot analysis. RESULTS: In the present study, we found that in the rat model of ARDS, Se@SiO(2) nanocomposites induced SOD and GSH indirectly to reduce ROS damage. The wet-to-dry weight ratio of lung was significantly decreased after MPS treatment compared with the control group, whereas the Se@SiO(2) did not affect the reduced wet-to-dry weight ratio of MPS. Se@SiO(2) also did not impair the effect of MPS on the reduction of inflammatory factors IL-1β and TNF-α, and on the alleviation of structural destruction. Furthermore, micro CT and histologic analysis confirmed that Se@SiO(2) significantly alleviate MPS-induced destruction of femoral head. Moreover, compared with MPS group, Se@SiO(2) could increase collagen II and aggrecan, and reduce the IL-1β level in the cartilage of femoral head. In addition, the biosafety of Se@SiO(2) in vitro and in vivo were supported by cell proliferation assay and histologic analysis of main organs from rat models. CONCLUSION: Se@SiO(2) nanocomposites have a protective effect in MPS-induced ONFH without influence on the therapeutic activity of MPS, suggesting the potential as effective drugs to avoid ONFH in MPS therapy. Dove Medical Press 2018-03-22 /pmc/articles/PMC5868597/ /pubmed/29606872 http://dx.doi.org/10.2147/IJN.S159776 Text en © 2018 Deng et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Deng, Guoying Dai, Chenyun Chen, Jinyuan Ji, Anqi Zhao, Jingpeng Zhai, Yue Kang, Yingjie Liu, Xijian Wang, Yin Wang, Qiugen Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
title | Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
title_full | Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
title_fullStr | Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
title_full_unstemmed | Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
title_short | Porous Se@SiO(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
title_sort | porous se@sio(2) nanocomposites protect the femoral head from methylprednisolone-induced osteonecrosis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868597/ https://www.ncbi.nlm.nih.gov/pubmed/29606872 http://dx.doi.org/10.2147/IJN.S159776 |
work_keys_str_mv | AT dengguoying poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT daichenyun poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT chenjinyuan poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT jianqi poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT zhaojingpeng poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT zhaiyue poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT kangyingjie poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT liuxijian poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT wangyin poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis AT wangqiugen poroussesio2nanocompositesprotectthefemoralheadfrommethylprednisoloneinducedosteonecrosis |