Cargando…

Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage

Current approaches for DNA extraction and fragmentation from mammalian spermatozoa provide several challenges for the investigation of the oxidative stress burden carried in the genome of male gametes. Indeed, the potential introduction of oxidative DNA damage induced by reactive oxygen species, red...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xavier, Miguel J., Nixon, Brett, Roman, Shaun D., Aitken, Robert John
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868848/ https://www.ncbi.nlm.nih.gov/pubmed/29579126 http://dx.doi.org/10.1371/journal.pone.0195003

Ejemplares similares

Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism
por: Houston, Brendan J., et al.
Publicado: (2019)

DNA damage in human spermatozoa; important contributor to mutagenesis in the offspring
por: Aitken, Robert John
Publicado: (2017)

Red light improves spermatozoa motility and does not induce oxidative DNA damage
por: Preece, Daryl, et al.
Publicado: (2017)

Antioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summer
por: Peña, Santiago T., et al.
Publicado: (2019)

Mitigating the Effects of Oxidative Sperm DNA Damage
por: Pini, Taylor, et al.
Publicado: (2020)

Mobile Phone Radiation Induces Reactive Oxygen Species Production and DNA Damage in Human Spermatozoa In Vitro
por: De Iuliis, Geoffry N., et al.
Publicado: (2009)

Glycerophospholipids protect stallion spermatozoa from oxidative damage in vitro
por: Medica, Ashlee J, et al.
Publicado: (2021)

The effects of vitamin C and menthone on acyclovir induced DNA damage in rat spermatozoa: An experimental study
por: Toghiani, Shima, et al.
Publicado: (2018)

Correction: Mobile Phone Radiation Induces Reactive Oxygen Species Production and DNA Damage in Human Spermatozoa In Vitro
por: De Iuliis, Geoffry N., et al.
Publicado: (2013)

Probing the Origins of 1,800 MHz Radio Frequency Electromagnetic Radiation Induced Damage in Mouse Immortalized Germ Cells and Spermatozoa in vitro
por: Houston, Brendan J., et al.
Publicado: (2018)

An Update on Oxidative Damage to Spermatozoa and Oocytes
por: Opuwari, Chinyerum S., et al.
Publicado: (2016)

Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage
por: Hourcade, Juan D, et al.
Publicado: (2010)

Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa
por: Raimondo, Salvatore, et al.
Publicado: (2014)

DNA fragmentation in spermatozoa: a historical review
por: Rex, A. S., et al.
Publicado: (2017)

Escherichia coli DNA ligase B may mitigate damage from oxidative stress
por: Bodine, Truston J., et al.
Publicado: (2017)

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage
por: Chiorcea-Paquim, Ana-Maria, et al.
Publicado: (2021)

Nuclear DNA Fragmentation in Boar Spermatozoa: Measurement Methods and Reproductive Performance Implications
por: Ausejo, Raquel, et al.
Publicado: (2022)

Loss of Nuclear/DNA Integrity in Mouse Epididymal Spermatozoa after Short-Term Exposure to Low Doses of Dibutyl Phthalate or Bisphenol AF and Its Mitigation by Oral Antioxidant Supplementation
por: Hug, Elisa, et al.
Publicado: (2023)

Human spermatozoa: revelations on the road to conception
por: Aitken, R. John
Publicado: (2013)

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword
por: Aitken, Robert J., et al.
Publicado: (2020)

Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa
por: Alqawasmeh, Odai AM, et al.
Publicado: (2020)

Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa
por: Tvrdá, Eva, et al.
Publicado: (2016)

Effect of statins on oxidative DNA damage in diabetic polyneuropathy
por: Carrillo-Ibarra, Sandra, et al.
Publicado: (2018)

Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method
por: Saylan, Aslihan, et al.
Publicado: (2016)

Levetiracetam mitigates doxorubicin-induced DNA and synaptic damage in neurons
por: Manchon, Jose Felix Moruno, et al.
Publicado: (2016)

Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility
por: Baker, Mark A, et al.
Publicado: (2005)

Novel Insights into DNA Methylation Features in Spermatozoa: Stability and Peculiarities
por: Krausz, Csilla, et al.
Publicado: (2012)

Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa
por: Rex, Anne Sofie, et al.
Publicado: (2019)

Seasonal Changes of Nuclear DNA Fragmentation in Boar Spermatozoa in Spain
por: Ausejo, Raquel, et al.
Publicado: (2021)

Generation of single stranded DNA with selective affinity to bovine spermatozoa
por: Vinod, Sivadasan Pathiyil, et al.
Publicado: (2021)

Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa
por: El Kamouh, Marina, et al.
Publicado: (2023)

Proteomic Landscape of Human Spermatozoa: Optimized Extraction Method and Application
por: Luo, Mengqi, et al.
Publicado: (2022)

Mechanistic Insight into the Regulation of Lipoxygenase-Driven Lipid Peroxidation Events in Human Spermatozoa and Their Impact on Male Fertility
por: Walters, Jessica L. H., et al.
Publicado: (2020)

Asciminib Mitigates DNA Damage Stress Signaling Induced by Cyclophosphamide in the Ovary
por: Mattiello, Luca, et al.
Publicado: (2021)

Electrochemical DNA Sensors for Detection of DNA Damage
por: Diculescu, Victor Constantin, et al.
Publicado: (2005)

Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease
por: Peña-Bautista, Carmen, et al.
Publicado: (2019)

DNA Profiling of Spermatozoa by Laser Capture Microdissection and Low Volume-PCR
por: Li, Cai-xia, et al.
Publicado: (2011)

p53 Protein Evaluation on Spermatozoa DNA in Fertile and Infertile Males
por: Raimondo, Salvatore, et al.
Publicado: (2019)

Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes
por: Martin, Jacinta H., et al.
Publicado: (2018)

Inflammation, oxidative DNA damage, and carcinogenesis.
por: Lewis, J G, et al.
Publicado: (1987)

Cannot write session to /tmp/vufind_sessions/sess_r9n2tqfiain09lnlhisrh2gmqv