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Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model

A multistage human gastrointestinal model was used to digest a polyphenol-rich potato extract containing chlorogenic acid, caffeic acid, ferulic acid, and rutin as the primary polyphenols, to assess for their microbial biotransformation and to measure changes in antioxidant capacity in up to 24 h of...

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Autores principales: Khairallah, Joelle, Sadeghi Ekbatan, Shima, Sabally, Kebba, Iskandar, Michèle M., Hussain, Raza, Nassar, Atef, Sleno, Lekha, Rodes, Laetitia, Prakash, Satya, Donnelly, Danielle J., Kubow, Stan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874529/
https://www.ncbi.nlm.nih.gov/pubmed/29558385
http://dx.doi.org/10.3390/antiox7030043
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author Khairallah, Joelle
Sadeghi Ekbatan, Shima
Sabally, Kebba
Iskandar, Michèle M.
Hussain, Raza
Nassar, Atef
Sleno, Lekha
Rodes, Laetitia
Prakash, Satya
Donnelly, Danielle J.
Kubow, Stan
author_facet Khairallah, Joelle
Sadeghi Ekbatan, Shima
Sabally, Kebba
Iskandar, Michèle M.
Hussain, Raza
Nassar, Atef
Sleno, Lekha
Rodes, Laetitia
Prakash, Satya
Donnelly, Danielle J.
Kubow, Stan
author_sort Khairallah, Joelle
collection PubMed
description A multistage human gastrointestinal model was used to digest a polyphenol-rich potato extract containing chlorogenic acid, caffeic acid, ferulic acid, and rutin as the primary polyphenols, to assess for their microbial biotransformation and to measure changes in antioxidant capacity in up to 24 h of digestion. The biotransformation of polyphenols was assessed by liquid chromatography–mass spectrometry. Antioxidant capacity was measured by the ferric reducing antioxidant power (FRAP) assay. Among the colonic reactors, parent (poly)phenols were detected in the ascending (AC), but not the transverse (TC) or descending (DC) colons. The most abundant microbial phenolic metabolites in all colonic reactors included derivatives of propionic acid, acetic acid, and benzoic acid. As compared to the baseline, an earlier increase in antioxidant capacity (T = 8 h) was seen in the stomach and small intestine vessels as compared to the AC (T = 16 h) and TC and DC (T = 24 h). The increase in antioxidant capacity observed in the DC and TC can be linked to the accumulation of microbial smaller-molecular-weight phenolic catabolites, as the parent polyphenolics had completely degraded in those vessels. The colonic microbial digestion of potato-based polyphenols could lead to improved colonic health, as this generates phenolic metabolites with significant antioxidant potential.
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spelling pubmed-58745292018-04-02 Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model Khairallah, Joelle Sadeghi Ekbatan, Shima Sabally, Kebba Iskandar, Michèle M. Hussain, Raza Nassar, Atef Sleno, Lekha Rodes, Laetitia Prakash, Satya Donnelly, Danielle J. Kubow, Stan Antioxidants (Basel) Article A multistage human gastrointestinal model was used to digest a polyphenol-rich potato extract containing chlorogenic acid, caffeic acid, ferulic acid, and rutin as the primary polyphenols, to assess for their microbial biotransformation and to measure changes in antioxidant capacity in up to 24 h of digestion. The biotransformation of polyphenols was assessed by liquid chromatography–mass spectrometry. Antioxidant capacity was measured by the ferric reducing antioxidant power (FRAP) assay. Among the colonic reactors, parent (poly)phenols were detected in the ascending (AC), but not the transverse (TC) or descending (DC) colons. The most abundant microbial phenolic metabolites in all colonic reactors included derivatives of propionic acid, acetic acid, and benzoic acid. As compared to the baseline, an earlier increase in antioxidant capacity (T = 8 h) was seen in the stomach and small intestine vessels as compared to the AC (T = 16 h) and TC and DC (T = 24 h). The increase in antioxidant capacity observed in the DC and TC can be linked to the accumulation of microbial smaller-molecular-weight phenolic catabolites, as the parent polyphenolics had completely degraded in those vessels. The colonic microbial digestion of potato-based polyphenols could lead to improved colonic health, as this generates phenolic metabolites with significant antioxidant potential. MDPI 2018-03-20 /pmc/articles/PMC5874529/ /pubmed/29558385 http://dx.doi.org/10.3390/antiox7030043 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khairallah, Joelle
Sadeghi Ekbatan, Shima
Sabally, Kebba
Iskandar, Michèle M.
Hussain, Raza
Nassar, Atef
Sleno, Lekha
Rodes, Laetitia
Prakash, Satya
Donnelly, Danielle J.
Kubow, Stan
Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model
title Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model
title_full Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model
title_fullStr Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model
title_full_unstemmed Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model
title_short Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model
title_sort microbial biotransformation of a polyphenol-rich potato extract affects antioxidant capacity in a simulated gastrointestinal model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874529/
https://www.ncbi.nlm.nih.gov/pubmed/29558385
http://dx.doi.org/10.3390/antiox7030043
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