Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies

To characterize a glutamine synthetase (GS)-based selection system, monoclonal antibody (mAb) producing recombinant CHO cell clones were generated by a single round of selection at various methionine sulfoximine (MSX) concentrations (0, 25, and 50 μM) using two different host cell lines (CHO-K1 and...

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Detalles Bibliográficos
Autores principales: Noh, Soo Min, Shin, Seunghyeon, Lee, Gyun Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876325/
https://www.ncbi.nlm.nih.gov/pubmed/29599455
http://dx.doi.org/10.1038/s41598-018-23720-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876325/
https://www.ncbi.nlm.nih.gov/pubmed/29599455
http://dx.doi.org/10.1038/s41598-018-23720-9

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