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Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers
Recent studies have shown that membrane proteins can be efficiently synthesized in vitro before spontaneously inserting into soluble nanoscale lipid bilayers called nanodiscs (NDs). In this paper, we present experimental details that allow a combination of in vitro translation of ion channels into c...
Autores principales: | Winterstein, Laura-Marie, Kukovetz, Kerri, Rauh, Oliver, Turman, Daniel L., Braun, Christian, Moroni, Anna, Schroeder, Indra, Thiel, Gerhard |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881443/ https://www.ncbi.nlm.nih.gov/pubmed/29487088 http://dx.doi.org/10.1085/jgp.201711904 |
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