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Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers

Recent studies have shown that membrane proteins can be efficiently synthesized in vitro before spontaneously inserting into soluble nanoscale lipid bilayers called nanodiscs (NDs). In this paper, we present experimental details that allow a combination of in vitro translation of ion channels into c...

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Detalles Bibliográficos
Autores principales: Winterstein, Laura-Marie, Kukovetz, Kerri, Rauh, Oliver, Turman, Daniel L., Braun, Christian, Moroni, Anna, Schroeder, Indra, Thiel, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881443/
https://www.ncbi.nlm.nih.gov/pubmed/29487088
http://dx.doi.org/10.1085/jgp.201711904