Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Komagataella phaffii (syn. Pichia pastoris) is one of the most commonly used host systems for recombinant protein expression. Achieving targeted genetic modifications had been hindered by low frequencies of homologous recombination (HR). Recently, a CRISPR/Cas9 genome editing system has been impleme...

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Detalles Bibliográficos
Autores principales: Weninger, Astrid, Fischer, Jasmin E., Raschmanová, Hana, Kniely, Claudia, Vogl, Thomas, Glieder, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887973/
https://www.ncbi.nlm.nih.gov/pubmed/29091307
http://dx.doi.org/10.1002/jcb.26474

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5887973/
https://www.ncbi.nlm.nih.gov/pubmed/29091307
http://dx.doi.org/10.1002/jcb.26474

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