Amplicon Competition Enables End‐Point Quantitation of Nucleic Acids Following Isothermal Amplification

It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We developed loop‐mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of “false” and “true” amplicons leads to order of magnitude quantitation by a si...

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Detalles Bibliográficos
Autores principales: Jiang, Yu Sherry, Stacy, Apollo, Whiteley, Marvin, Ellington, Andrew D., Bhadra, Sanchita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890436/
https://www.ncbi.nlm.nih.gov/pubmed/28628741
http://dx.doi.org/10.1002/cbic.201700317

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890436/
https://www.ncbi.nlm.nih.gov/pubmed/28628741
http://dx.doi.org/10.1002/cbic.201700317

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