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Amplicon Competition Enables End‐Point Quantitation of Nucleic Acids Following Isothermal Amplification
It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We developed loop‐mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of “false” and “true” amplicons leads to order of magnitude quantitation by a si...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5890436/ https://www.ncbi.nlm.nih.gov/pubmed/28628741 http://dx.doi.org/10.1002/cbic.201700317 |