Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

This protocol demonstrates a method for obtaining high yield and viability for mouse hepatocytes and sinusoidal endothelial cells (SECs) suitable for culturing or for obtaining cell lysates. In this protocol, the portal vein is used as the site for catheterization, rather than the vena cava, as this...

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Detalles Bibliográficos
Autores principales: Cabral, Fatima, Miller, Colton M., Kudrna, Katrina M., Hass, Blake E., Daubendiek, Jocelyn G., Kellar, Brianna M., Harris, Edward N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894826/
https://www.ncbi.nlm.nih.gov/pubmed/29553556
http://dx.doi.org/10.3791/56993

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894826/
https://www.ncbi.nlm.nih.gov/pubmed/29553556
http://dx.doi.org/10.3791/56993

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