Strategies for enumeration of circulating microvesicles on a conventional flow cytometer: Counting beads and scatter parameters

Enumeration of circulating microvesicles (MVs) by conventional flow cytometry is accomplished by the addition of a known amount of counting beads and calculated from the formula: MV/μl = (MV count/bead count) × final bead concentration. We sought to optimize each variable in the equation by determin...

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Detalles Bibliográficos
Autores principales: Alkhatatbeh, Mohammad J, Enjeti, Anoop K, Baqar, Sara, Ekinci, Elif I, Liu, Dorothy, Thorne, Rick F, Lincz, Lisa F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894907/
https://www.ncbi.nlm.nih.gov/pubmed/29662552
http://dx.doi.org/10.1177/1849454418766966