Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia

Inducible DNA recombination of floxed alleles in vivo by liver metabolites of tamoxifen (TAM) is an important tool to study gene functions. Here, we describe protocols for optimal DNA recombination in astrocytes, based on the GLAST-Cre(ERT2)/loxP system. In addition, we demonstrate that quantificati...

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Detalles Bibliográficos
Autores principales: Jahn, Hannah M., Kasakow, Carmen V., Helfer, Andreas, Michely, Julian, Verkhratsky, Alexei, Maurer, Hans H., Scheller, Anja, Kirchhoff, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897555/
https://www.ncbi.nlm.nih.gov/pubmed/29651133
http://dx.doi.org/10.1038/s41598-018-24085-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897555/
https://www.ncbi.nlm.nih.gov/pubmed/29651133
http://dx.doi.org/10.1038/s41598-018-24085-9

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