Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing technology to functional genetics and human therapeutic applications. Here, we show that incorporation of next-generation bridged nucleic acids (2′,4′-BNA(NC)[N-Me]) as well as locked nucleic acids (LNA) at specifi...

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Detalles Bibliográficos
Autores principales: Cromwell, Christopher R., Sung, Keewon, Park, Jinho, Krysler, Amanda R., Jovel, Juan, Kim, Seong Keun, Hubbard, Basil P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899152/
https://www.ncbi.nlm.nih.gov/pubmed/29654299
http://dx.doi.org/10.1038/s41467-018-03927-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899152/
https://www.ncbi.nlm.nih.gov/pubmed/29654299
http://dx.doi.org/10.1038/s41467-018-03927-0

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