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Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing technology to functional genetics and human therapeutic applications. Here, we show that incorporation of next-generation bridged nucleic acids (2′,4′-BNA(NC)[N-Me]) as well as locked nucleic acids (LNA) at specifi...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899152/ https://www.ncbi.nlm.nih.gov/pubmed/29654299 http://dx.doi.org/10.1038/s41467-018-03927-0 |