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Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis
Almost all cells require thiamin, vitamin B1 (B1), which is synthesized via the coupling of thiazole and pyrimidine precursors. Here we demonstrate that 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET) is a useful in vivo B1 precursor for representatives of ubiquitous marine picoeuk...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899164/ https://www.ncbi.nlm.nih.gov/pubmed/29654239 http://dx.doi.org/10.1038/s41598-018-24321-2 |
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author | Paerl, Ryan W. Bertrand, Erin M. Rowland, Elden Schatt, Phillippe Mehiri, Mohamed Niehaus, Thomas D. Hanson, Andrew D. Riemann, Lasse Bouget, Francois-Yves |
author_facet | Paerl, Ryan W. Bertrand, Erin M. Rowland, Elden Schatt, Phillippe Mehiri, Mohamed Niehaus, Thomas D. Hanson, Andrew D. Riemann, Lasse Bouget, Francois-Yves |
author_sort | Paerl, Ryan W. |
collection | PubMed |
description | Almost all cells require thiamin, vitamin B1 (B1), which is synthesized via the coupling of thiazole and pyrimidine precursors. Here we demonstrate that 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET) is a useful in vivo B1 precursor for representatives of ubiquitous marine picoeukaryotic phytoplankton and Escherichia coli – drawing attention to cHET as a valuable exogenous micronutrient for microorganisms with ecological, industrial, and biomedical value. Comparative utilization experiments with the terrestrial plant Arabidopsis thaliana revealed that it can also use exogenous cHET, but notably, picoeukaryotic marine phytoplankton and E. coli were adapted to grow on low (picomolar) concentrations of exogenous cHET. Our results call for the modification of the conventional B1 biosynthesis model to incorporate cHET as a key precursor for B1 biosynthesis in two domains of life, and for consideration of cHET as a microbial micronutrient currency modulating marine primary productivity and community interactions in human gut-hosted microbiomes. |
format | Online Article Text |
id | pubmed-5899164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58991642018-04-20 Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis Paerl, Ryan W. Bertrand, Erin M. Rowland, Elden Schatt, Phillippe Mehiri, Mohamed Niehaus, Thomas D. Hanson, Andrew D. Riemann, Lasse Bouget, Francois-Yves Sci Rep Article Almost all cells require thiamin, vitamin B1 (B1), which is synthesized via the coupling of thiazole and pyrimidine precursors. Here we demonstrate that 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET) is a useful in vivo B1 precursor for representatives of ubiquitous marine picoeukaryotic phytoplankton and Escherichia coli – drawing attention to cHET as a valuable exogenous micronutrient for microorganisms with ecological, industrial, and biomedical value. Comparative utilization experiments with the terrestrial plant Arabidopsis thaliana revealed that it can also use exogenous cHET, but notably, picoeukaryotic marine phytoplankton and E. coli were adapted to grow on low (picomolar) concentrations of exogenous cHET. Our results call for the modification of the conventional B1 biosynthesis model to incorporate cHET as a key precursor for B1 biosynthesis in two domains of life, and for consideration of cHET as a microbial micronutrient currency modulating marine primary productivity and community interactions in human gut-hosted microbiomes. Nature Publishing Group UK 2018-04-13 /pmc/articles/PMC5899164/ /pubmed/29654239 http://dx.doi.org/10.1038/s41598-018-24321-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Paerl, Ryan W. Bertrand, Erin M. Rowland, Elden Schatt, Phillippe Mehiri, Mohamed Niehaus, Thomas D. Hanson, Andrew D. Riemann, Lasse Bouget, Francois-Yves Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
title | Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
title_full | Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
title_fullStr | Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
title_full_unstemmed | Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
title_short | Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
title_sort | carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899164/ https://www.ncbi.nlm.nih.gov/pubmed/29654239 http://dx.doi.org/10.1038/s41598-018-24321-2 |
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