Lipofection of siRNA into bovine 8-16-cell stage embryos using zona removal and the well-of-the-well culture system

Bovine preimplantation embryos exhibit dramatic biological changes between before and after the 8-16-cell stage. Here we report a simple lipofection method to transfect siRNA into bovine 8-16-cell stage embryos using zona removal and the well-of-the-well (WOW) culture system. Bovine one-cell embryos...

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Detalles Bibliográficos
Autores principales: IKEDA, Shuntaro, SUGIMOTO, Miki, KUME, Shinichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2018
Materias:
Technology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902909/
https://www.ncbi.nlm.nih.gov/pubmed/29353869
http://dx.doi.org/10.1262/jrd.2017-137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902909/
https://www.ncbi.nlm.nih.gov/pubmed/29353869
http://dx.doi.org/10.1262/jrd.2017-137

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