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Lipofection of siRNA into bovine 8-16-cell stage embryos using zona removal and the well-of-the-well culture system
Bovine preimplantation embryos exhibit dramatic biological changes between before and after the 8-16-cell stage. Here we report a simple lipofection method to transfect siRNA into bovine 8-16-cell stage embryos using zona removal and the well-of-the-well (WOW) culture system. Bovine one-cell embryos...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Society for Reproduction and Development
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902909/ https://www.ncbi.nlm.nih.gov/pubmed/29353869 http://dx.doi.org/10.1262/jrd.2017-137 |