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Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays

BACKGROUND: Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols in routine diagnosis. To date, there are only...

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Detalles Bibliográficos
Autores principales: Abras, Alba, Ballart, Cristina, Llovet, Teresa, Roig, Carme, Gutiérrez, Cristina, Tebar, Silvia, Berenguer, Pere, Pinazo, María-Jesús, Posada, Elizabeth, Gascón, Joaquim, Schijman, Alejandro G., Gállego, Montserrat, Muñoz, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903661/
https://www.ncbi.nlm.nih.gov/pubmed/29664973
http://dx.doi.org/10.1371/journal.pone.0195738