Ultrastructural localisation of protein interactions using conditionally stable nanobodies

We describe the development and application of a suite of modular tools for high-resolution detection of proteins and intracellular protein complexes by electron microscopy (EM). Conditionally stable GFP- and mCherry-binding nanobodies (termed csGBP and csChBP, respectively) are characterized using...

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Detalles Bibliográficos
Autores principales: Ariotti, Nicholas, Rae, James, Giles, Nichole, Martel, Nick, Sierecki, Emma, Gambin, Yann, Hall, Thomas E., Parton, Robert G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903671/
https://www.ncbi.nlm.nih.gov/pubmed/29621251
http://dx.doi.org/10.1371/journal.pbio.2005473

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903671/
https://www.ncbi.nlm.nih.gov/pubmed/29621251
http://dx.doi.org/10.1371/journal.pbio.2005473

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