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Ultrastructural localisation of protein interactions using conditionally stable nanobodies

We describe the development and application of a suite of modular tools for high-resolution detection of proteins and intracellular protein complexes by electron microscopy (EM). Conditionally stable GFP- and mCherry-binding nanobodies (termed csGBP and csChBP, respectively) are characterized using...

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Detalles Bibliográficos
Autores principales:	Ariotti, Nicholas, Rae, James, Giles, Nichole, Martel, Nick, Sierecki, Emma, Gambin, Yann, Hall, Thomas E., Parton, Robert G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Methods and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903671/ https://www.ncbi.nlm.nih.gov/pubmed/29621251 http://dx.doi.org/10.1371/journal.pbio.2005473

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