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Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage

Phospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position sn1 whereas the sn2-FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsatura...

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Autores principales: Manni, Marco M, Tiberti, Marion L, Pagnotta, Sophie, Barelli, Hélène, Gautier, Romain, Antonny, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903860/
https://www.ncbi.nlm.nih.gov/pubmed/29543154
http://dx.doi.org/10.7554/eLife.34394
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author Manni, Marco M
Tiberti, Marion L
Pagnotta, Sophie
Barelli, Hélène
Gautier, Romain
Antonny, Bruno
author_facet Manni, Marco M
Tiberti, Marion L
Pagnotta, Sophie
Barelli, Hélène
Gautier, Romain
Antonny, Bruno
author_sort Manni, Marco M
collection PubMed
description Phospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position sn1 whereas the sn2-FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsaturation on membrane flexibility and fission is fragmentary. Here, we provide a comprehensive view of the effects of the FA profile of phospholipids on membrane vesiculation by dynamin and endophilin. Coupled to simulations, this analysis indicates that: (i) phospholipids with two polyunsaturated FAs make membranes prone to vesiculation but highly permeable; (ii) asymmetric sn1-saturated-sn2-polyunsaturated phospholipids provide a tradeoff between efficient membrane vesiculation and low membrane permeability; (iii) When incorporated into phospholipids, docosahexaenoic acid (DHA; omega-3) makes membranes more deformable than arachidonic acid (omega-6). These results suggest an explanation for the abundance of sn1-saturated-sn2-DHA phospholipids in synaptic membranes and for the importance of the omega-6/omega-3 ratio on neuronal functions.
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spelling pubmed-59038602018-04-18 Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage Manni, Marco M Tiberti, Marion L Pagnotta, Sophie Barelli, Hélène Gautier, Romain Antonny, Bruno eLife Biochemistry and Chemical Biology Phospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position sn1 whereas the sn2-FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsaturation on membrane flexibility and fission is fragmentary. Here, we provide a comprehensive view of the effects of the FA profile of phospholipids on membrane vesiculation by dynamin and endophilin. Coupled to simulations, this analysis indicates that: (i) phospholipids with two polyunsaturated FAs make membranes prone to vesiculation but highly permeable; (ii) asymmetric sn1-saturated-sn2-polyunsaturated phospholipids provide a tradeoff between efficient membrane vesiculation and low membrane permeability; (iii) When incorporated into phospholipids, docosahexaenoic acid (DHA; omega-3) makes membranes more deformable than arachidonic acid (omega-6). These results suggest an explanation for the abundance of sn1-saturated-sn2-DHA phospholipids in synaptic membranes and for the importance of the omega-6/omega-3 ratio on neuronal functions. eLife Sciences Publications, Ltd 2018-03-15 /pmc/articles/PMC5903860/ /pubmed/29543154 http://dx.doi.org/10.7554/eLife.34394 Text en © 2018, Manni et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Manni, Marco M
Tiberti, Marion L
Pagnotta, Sophie
Barelli, Hélène
Gautier, Romain
Antonny, Bruno
Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
title Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
title_full Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
title_fullStr Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
title_full_unstemmed Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
title_short Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
title_sort acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903860/
https://www.ncbi.nlm.nih.gov/pubmed/29543154
http://dx.doi.org/10.7554/eLife.34394
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