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Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition
Mitochondrial dysfunction is prevalent in the aging gastrointestinal tract. We investigated whether mitochondrial function in aging colonic crypts and exercise influences microbial gut communities in mice. Twelve PolgA(mut)/(mut) mice were randomly divided into a sedentary and exercise group at 4 mo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905657/ https://www.ncbi.nlm.nih.gov/pubmed/29045670 http://dx.doi.org/10.1093/gerona/glx197 |
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author | Houghton, David Stewart, Christopher J Stamp, Craig Nelson, Andrew Aj ami, Nadim J Petrosino, Joseph F Wipat, Anil Trenell, Michael I Turnbull, Douglass M Greaves, Laura C |
author_facet | Houghton, David Stewart, Christopher J Stamp, Craig Nelson, Andrew Aj ami, Nadim J Petrosino, Joseph F Wipat, Anil Trenell, Michael I Turnbull, Douglass M Greaves, Laura C |
author_sort | Houghton, David |
collection | PubMed |
description | Mitochondrial dysfunction is prevalent in the aging gastrointestinal tract. We investigated whether mitochondrial function in aging colonic crypts and exercise influences microbial gut communities in mice. Twelve PolgA(mut)/(mut) mice were randomly divided into a sedentary and exercise group at 4 months. Seven-aged matched PolgA(+/+) mice remained sedentary throughout. Stool samples were collected at 4, 7, and 11 months, and bacterial profiling was achieved through 16S rRNA sequencing profiling. Mitochondrial enzyme activity was assessed in colonic epithelial crypts at 11 months for PolgA(mut)/(mut) and PolgA(+/+) mice. Sedentary and exercised PolgA(mut)/(mut) mice had significantly higher levels of mitochondrial dysfunction than PolgA(+/+) mice (78%, 77%, and 1% of crypts, respectively). Bacterial profiles of sedentary PolgA(mut)/(mut) mice were significantly different from the sedentary PolgA(+/+) mice, with increases in Lactobacillus and Mycoplasma, and decreases in Alistipes, Odoribacter, Anaeroplasma, Rikenella, Parabacteroides, and Allobaculum in the PolgA(mut)/(mut) mice. Exercise did not have any impact upon gut mitochondrial dysfunction; however, exercise did increase gut microbiota diversity and significantly increased bacterial genera Mucispirillum and Desulfovibrio. Mitochondrial dysfunction is associated with changes in the gut microbiota. Endurance exercise moderated some of these changes, establishing that environmental factors can influence gut microbiota, despite mitochondrial dysfunction. |
format | Online Article Text |
id | pubmed-5905657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59056572018-04-23 Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition Houghton, David Stewart, Christopher J Stamp, Craig Nelson, Andrew Aj ami, Nadim J Petrosino, Joseph F Wipat, Anil Trenell, Michael I Turnbull, Douglass M Greaves, Laura C J Gerontol A Biol Sci Med Sci The Journal of Gerontology: Biological Sciences Mitochondrial dysfunction is prevalent in the aging gastrointestinal tract. We investigated whether mitochondrial function in aging colonic crypts and exercise influences microbial gut communities in mice. Twelve PolgA(mut)/(mut) mice were randomly divided into a sedentary and exercise group at 4 months. Seven-aged matched PolgA(+/+) mice remained sedentary throughout. Stool samples were collected at 4, 7, and 11 months, and bacterial profiling was achieved through 16S rRNA sequencing profiling. Mitochondrial enzyme activity was assessed in colonic epithelial crypts at 11 months for PolgA(mut)/(mut) and PolgA(+/+) mice. Sedentary and exercised PolgA(mut)/(mut) mice had significantly higher levels of mitochondrial dysfunction than PolgA(+/+) mice (78%, 77%, and 1% of crypts, respectively). Bacterial profiles of sedentary PolgA(mut)/(mut) mice were significantly different from the sedentary PolgA(+/+) mice, with increases in Lactobacillus and Mycoplasma, and decreases in Alistipes, Odoribacter, Anaeroplasma, Rikenella, Parabacteroides, and Allobaculum in the PolgA(mut)/(mut) mice. Exercise did not have any impact upon gut mitochondrial dysfunction; however, exercise did increase gut microbiota diversity and significantly increased bacterial genera Mucispirillum and Desulfovibrio. Mitochondrial dysfunction is associated with changes in the gut microbiota. Endurance exercise moderated some of these changes, establishing that environmental factors can influence gut microbiota, despite mitochondrial dysfunction. Oxford University Press 2018-04 2017-10-16 /pmc/articles/PMC5905657/ /pubmed/29045670 http://dx.doi.org/10.1093/gerona/glx197 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of The Gerontological Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | The Journal of Gerontology: Biological Sciences Houghton, David Stewart, Christopher J Stamp, Craig Nelson, Andrew Aj ami, Nadim J Petrosino, Joseph F Wipat, Anil Trenell, Michael I Turnbull, Douglass M Greaves, Laura C Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition |
title | Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition |
title_full | Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition |
title_fullStr | Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition |
title_full_unstemmed | Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition |
title_short | Impact of Age-Related Mitochondrial Dysfunction and Exercise on Intestinal Microbiota Composition |
title_sort | impact of age-related mitochondrial dysfunction and exercise on intestinal microbiota composition |
topic | The Journal of Gerontology: Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905657/ https://www.ncbi.nlm.nih.gov/pubmed/29045670 http://dx.doi.org/10.1093/gerona/glx197 |
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