Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for e...

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Detalles Bibliográficos
Autores principales: Zhu, Dongjie, Wang, Xiangxi, Fang, Qianglin, Van Etten, James L, Rossmann, Michael G, Rao, Zihe, Zhang, Xinzheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908801/
https://www.ncbi.nlm.nih.gov/pubmed/29674632
http://dx.doi.org/10.1038/s41467-018-04051-9
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author Zhu, Dongjie
Wang, Xiangxi
Fang, Qianglin
Van Etten, James L
Rossmann, Michael G
Rao, Zihe
Zhang, Xinzheng
author_facet Zhu, Dongjie
Wang, Xiangxi
Fang, Qianglin
Van Etten, James L
Rossmann, Michael G
Rao, Zihe
Zhang, Xinzheng
author_sort Zhu, Dongjie
collection PubMed
description The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.
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spelling pubmed-59088012018-04-23 Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions Zhu, Dongjie Wang, Xiangxi Fang, Qianglin Van Etten, James L Rossmann, Michael G Rao, Zihe Zhang, Xinzheng Nat Commun Article The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes. Nature Publishing Group UK 2018-04-19 /pmc/articles/PMC5908801/ /pubmed/29674632 http://dx.doi.org/10.1038/s41467-018-04051-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhu, Dongjie
Wang, Xiangxi
Fang, Qianglin
Van Etten, James L
Rossmann, Michael G
Rao, Zihe
Zhang, Xinzheng
Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_full Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_fullStr Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_full_unstemmed Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_short Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
title_sort pushing the resolution limit by correcting the ewald sphere effect in single-particle cryo-em reconstructions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908801/
https://www.ncbi.nlm.nih.gov/pubmed/29674632
http://dx.doi.org/10.1038/s41467-018-04051-9
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