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The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis

Next-generation sequencing approaches used to characterize microbial communities are subject to technical caveats that can lead to major distortion of acquired data. Determining the optimal sample handling protocol is essential to minimize the bias for different sample types. Using a mock community...

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Detalles Bibliográficos
Autores principales: Hallmaier-Wacker, Luisa K., Lueert, Simone, Roos, Christian, Knauf, Sascha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908915/
https://www.ncbi.nlm.nih.gov/pubmed/29674641
http://dx.doi.org/10.1038/s41598-018-24573-y