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The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis
Next-generation sequencing approaches used to characterize microbial communities are subject to technical caveats that can lead to major distortion of acquired data. Determining the optimal sample handling protocol is essential to minimize the bias for different sample types. Using a mock community...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908915/ https://www.ncbi.nlm.nih.gov/pubmed/29674641 http://dx.doi.org/10.1038/s41598-018-24573-y |