Cargando…

Enabling multiplexed testing of pooled donor cells through whole-genome sequencing

We describe a method that enables the multiplex screening of a pool of many different donor cell lines. Our method accurately predicts each donor proportion from the pool without requiring the use of unique DNA barcodes as markers of donor identity. Instead, we take advantage of common single nucleo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chan, Yingleong, Chan, Ying Kai, Goodman, Daniel B., Guo, Xiaoge, Chavez, Alejandro, Lim, Elaine T., Church, George M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909281/ https://www.ncbi.nlm.nih.gov/pubmed/29673390 http://dx.doi.org/10.1186/s13073-018-0541-6

Ejemplares similares

Reliable multiplex generation of pooled induced pluripotent stem cells
por: Smullen, Molly, et al.
Publicado: (2023)

Accurate analysis of genuine CRISPR editing events with ampliCan
por: Labun, Kornel, et al.
Publicado: (2019)

Optimizing complex phenotypes through model-guided multiplex genome engineering
por: Kuznetsov, Gleb, et al.
Publicado: (2017)

Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples
por: Smith, Andrew M., et al.
Publicado: (2010)

Shortcut barcoding and early pooling for scalable multiplex single-cell reduced-representation CpG methylation sequencing at single nucleotide resolution
por: Mai, Liyao, et al.
Publicado: (2023)

Multiplex sequencing of pooled mitochondrial genomes—a crucial step toward biodiversity analysis using mito-metagenomics
por: Tang, Min, et al.
Publicado: (2014)

High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR/Cas9 in yeast
por: Guo, Xiaoge, et al.
Publicado: (2018)

Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics
por: Stoeckius, Marlon, et al.
Publicado: (2018)

Identification of novel causative genes determining the complex trait of high ethanol tolerance in yeast using pooled-segregant whole-genome sequence analysis
por: Swinnen, Steve, et al.
Publicado: (2012)

Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing
por: Ståhlberg, Anders, et al.
Publicado: (2016)

Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria
por: Wang, Xue, et al.
Publicado: (2021)

Pooled Sequencing and Rare Variant Association Tests for Identifying the Determinants of Emerging Drug Resistance in Malaria Parasites
por: Cheeseman, Ian H., et al.
Publicado: (2015)

Maximum Likelihood Estimation of Frequencies of Known Haplotypes from Pooled Sequence Data
por: Kessner, Darren, et al.
Publicado: (2013)

Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
por: Dahl, Fredrik, et al.
Publicado: (2005)

Multiplexed Target Enrichment Enables Efficient and In-Depth Analysis of Antimicrobial Resistome in Metagenomes
por: Li, Yiming, et al.
Publicado: (2022)

Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture
por: Rohland, Nadin, et al.
Publicado: (2012)

Optimizing sparse sequencing of single cells for highly multiplex copy number profiling
por: Baslan, Timour, et al.
Publicado: (2015)

A multiplexed, three-dimensional pooling and next-generation sequencing strategy for creating barcoded mutant arrays: construction of a Schizosaccharomyces pombe transposon insertion library
por: Li, Yanhui, et al.
Publicado: (2022)

Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection
por: Carr, Peter A., et al.
Publicado: (2012)

Detection of structural mosaicism from targeted and whole-genome sequencing data
por: King, Daniel A., et al.
Publicado: (2017)

Pooling across cells to normalize single-cell RNA sequencing data with many zero counts
por: L. Lun, Aaron T., et al.
Publicado: (2016)

Pooled protein tagging, cellular imaging, and in situ sequencing for monitoring drug action in real time
por: Reicher, Andreas, et al.
Publicado: (2020)

Mutation discovery in mice by whole exome sequencing
por: Fairfield, Heather, et al.
Publicado: (2011)

Mutation mapping and identification by whole-genome sequencing
por: Leshchiner, Ignaty, et al.
Publicado: (2012)

Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling
por: Boyle, Patrick, et al.
Publicado: (2012)

DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes
por: Kuno, Akihiro, et al.
Publicado: (2022)

High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing
por: Sims, David, et al.
Publicado: (2011)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis
por: Niranjan, Tejasvi S, et al.
Publicado: (2011)

Hierarchical Interleaved Bloom Filter: enabling ultrafast, approximate sequence queries
por: Mehringer, Svenja, et al.
Publicado: (2023)

Identification of species by multiplex analysis of variable-length sequences
por: Pereira, Filipe, et al.
Publicado: (2010)

Whole-genome bisulfite sequencing with improved accuracy and cost
por: Suzuki, Masako, et al.
Publicado: (2018)

Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology
por: Cronn, Richard, et al.
Publicado: (2008)

PRAM: a novel pooling approach for discovering intergenic transcripts from large-scale RNA sequencing experiments
por: Liu, Peng, et al.
Publicado: (2020)

Inferring Demography from Runs of Homozygosity in Whole-Genome Sequence, with Correction for Sequence Errors
por: MacLeod, Iona M., et al.
Publicado: (2013)

An accurate clone-based haplotyping method by overlapping pool sequencing
por: Li, Cheng, et al.
Publicado: (2016)

Multiplexed direct genomic selection (MDiGS): a pooled BAC capture approach for highly accurate CNV and SNP/INDEL detection
por: Alvarado, David M., et al.
Publicado: (2014)

Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium
por: Li, Cheng-Lin Frank, et al.
Publicado: (2016)

Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering
por: Lajoie, M. J., et al.
Publicado: (2012)

Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination
por: Aggarwal, Dinesh, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_cgffrl58use07ho7kjkm6q28ia