CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells

CRISPR/Cas9-based genome editing offers the possibility to knock out almost any gene of interest in an affordable and simple manner. The most common strategy is the introduction of a frameshift into the open reading frame (ORF) of the target gene which truncates the coding sequence (CDS) and targets...

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Detalles Bibliográficos
Autores principales: Reber, Stefan, Mechtersheimer, Jonas, Nasif, Sofia, Benitez, Julio Aguila, Colombo, Martino, Domanski, Michal, Jutzi, Daniel, Hedlund, Eva, Ruepp, Marc-David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909934/
https://www.ncbi.nlm.nih.gov/pubmed/29167381
http://dx.doi.org/10.1091/mbc.E17-05-0288

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909934/
https://www.ncbi.nlm.nih.gov/pubmed/29167381
http://dx.doi.org/10.1091/mbc.E17-05-0288

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