The ISG15 Conjugation System

ISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling (IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the transcriptional level by IFN-α...

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Detalles Bibliográficos
Autores principales: Durfee, Larissa A., Huibregtse, Jon M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912894/
https://www.ncbi.nlm.nih.gov/pubmed/22350882
http://dx.doi.org/10.1007/978-1-61779-474-2_9
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author Durfee, Larissa A.
Huibregtse, Jon M.
author_facet Durfee, Larissa A.
Huibregtse, Jon M.
author_sort Durfee, Larissa A.
collection PubMed
description ISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling (IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes and modifies target proteins in a cotranslational manner. Here, we describe the expression of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the methods for fractionation of Herc5 with polysomes.
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spelling pubmed-59128942018-04-23 The ISG15 Conjugation System Durfee, Larissa A. Huibregtse, Jon M. Ubiquitin Family Modifiers and the Proteasome Article ISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling (IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes and modifies target proteins in a cotranslational manner. Here, we describe the expression of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the methods for fractionation of Herc5 with polysomes. 2011-11-15 /pmc/articles/PMC5912894/ /pubmed/22350882 http://dx.doi.org/10.1007/978-1-61779-474-2_9 Text en © Springer Science+Business Media, LLC 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Durfee, Larissa A.
Huibregtse, Jon M.
The ISG15 Conjugation System
title The ISG15 Conjugation System
title_full The ISG15 Conjugation System
title_fullStr The ISG15 Conjugation System
title_full_unstemmed The ISG15 Conjugation System
title_short The ISG15 Conjugation System
title_sort isg15 conjugation system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912894/
https://www.ncbi.nlm.nih.gov/pubmed/22350882
http://dx.doi.org/10.1007/978-1-61779-474-2_9
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