True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

The complex patterns of gene expression in metazoans are controlled by selective binding of transcription factors (TFs) to regulatory DNA. To improve the quantitative understanding of this process, we have developed a novel method that uses fluorescence anisotropy measurements in a controlled delive...

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Detalles Bibliográficos
Autores principales: Jung, Christophe, Bandilla, Peter, von Reutern, Marc, Schnepf, Max, Rieder, Susanne, Unnerstall, Ulrich, Gaul, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913336/
https://www.ncbi.nlm.nih.gov/pubmed/29686282
http://dx.doi.org/10.1038/s41467-018-03977-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913336/
https://www.ncbi.nlm.nih.gov/pubmed/29686282
http://dx.doi.org/10.1038/s41467-018-03977-4

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