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True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
The complex patterns of gene expression in metazoans are controlled by selective binding of transcription factors (TFs) to regulatory DNA. To improve the quantitative understanding of this process, we have developed a novel method that uses fluorescence anisotropy measurements in a controlled delive...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913336/ https://www.ncbi.nlm.nih.gov/pubmed/29686282 http://dx.doi.org/10.1038/s41467-018-03977-4 |