Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice

Persistence of Mycobacterium tuberculosis within human bone marrow stem cells has been identified as a potential bacterial niche during latent tuberculosis. Using a murine model of tuberculosis, we show here that bone marrow stem and progenitor cells containing M. tuberculosis propagated tuberculosi...

Descripción completa

Detalles Bibliográficos
Autores principales: Reece, Stephen T, Vogelzang, Alexis, Tornack, Julia, Bauer, Wolfgang, Zedler, Ulrike, Schommer-Leitner, Sandra, Stingl, Georg, Melchers, Fritz, Kaufmann, Stefan H E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Major Articles and Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913604/
https://www.ncbi.nlm.nih.gov/pubmed/29471332
http://dx.doi.org/10.1093/infdis/jiy041
_version_ 1783316571837431808
author Reece, Stephen T
Vogelzang, Alexis
Tornack, Julia
Bauer, Wolfgang
Zedler, Ulrike
Schommer-Leitner, Sandra
Stingl, Georg
Melchers, Fritz
Kaufmann, Stefan H E
author_facet Reece, Stephen T
Vogelzang, Alexis
Tornack, Julia
Bauer, Wolfgang
Zedler, Ulrike
Schommer-Leitner, Sandra
Stingl, Georg
Melchers, Fritz
Kaufmann, Stefan H E
author_sort Reece, Stephen T
collection PubMed
description Persistence of Mycobacterium tuberculosis within human bone marrow stem cells has been identified as a potential bacterial niche during latent tuberculosis. Using a murine model of tuberculosis, we show here that bone marrow stem and progenitor cells containing M. tuberculosis propagated tuberculosis when transferred to naive mice, given that both transferred cells and recipient mice were unable to express inducible nitric oxide synthase, which mediates killing of intracellular bacteria via nitric oxide. Our findings suggest that bone marrow stem and progenitor cells containing M. tuberculosis propagate hallmarks of disease if nitric oxide-mediated killing of bacteria is defective.
format Online
Article
Text
id pubmed-5913604
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-59136042018-04-30 Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice Reece, Stephen T Vogelzang, Alexis Tornack, Julia Bauer, Wolfgang Zedler, Ulrike Schommer-Leitner, Sandra Stingl, Georg Melchers, Fritz Kaufmann, Stefan H E J Infect Dis Major Articles and Brief Reports Persistence of Mycobacterium tuberculosis within human bone marrow stem cells has been identified as a potential bacterial niche during latent tuberculosis. Using a murine model of tuberculosis, we show here that bone marrow stem and progenitor cells containing M. tuberculosis propagated tuberculosis when transferred to naive mice, given that both transferred cells and recipient mice were unable to express inducible nitric oxide synthase, which mediates killing of intracellular bacteria via nitric oxide. Our findings suggest that bone marrow stem and progenitor cells containing M. tuberculosis propagate hallmarks of disease if nitric oxide-mediated killing of bacteria is defective. Oxford University Press 2018-05-15 2018-02-17 /pmc/articles/PMC5913604/ /pubmed/29471332 http://dx.doi.org/10.1093/infdis/jiy041 Text en © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Major Articles and Brief Reports
Reece, Stephen T
Vogelzang, Alexis
Tornack, Julia
Bauer, Wolfgang
Zedler, Ulrike
Schommer-Leitner, Sandra
Stingl, Georg
Melchers, Fritz
Kaufmann, Stefan H E
Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice
title Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice
title_full Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice
title_fullStr Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice
title_full_unstemmed Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice
title_short Mycobacterium tuberculosis-Infected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice
title_sort mycobacterium tuberculosis-infected hematopoietic stem and progenitor cells unable to express inducible nitric oxide synthase propagate tuberculosis in mice
topic Major Articles and Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913604/
https://www.ncbi.nlm.nih.gov/pubmed/29471332
http://dx.doi.org/10.1093/infdis/jiy041
work_keys_str_mv AT reecestephent mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT vogelzangalexis mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT tornackjulia mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT bauerwolfgang mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT zedlerulrike mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT schommerleitnersandra mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT stinglgeorg mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT melchersfritz mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice
AT kaufmannstefanhe mycobacteriumtuberculosisinfectedhematopoieticstemandprogenitorcellsunabletoexpressinduciblenitricoxidesynthasepropagatetuberculosisinmice

Ejemplares similares