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A simple antegrade perfusion method for isolating viable single cardiomyocytes from neonatal to aged mice

The aim of this study was to establish a simple and reproducible antegrade perfusion method for isolating single viable mouse heart cells and to determine the standard practical protocols that are appropriate for mice of various ages. Antegrade perfusion was performed by injecting perfusate from nea...

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Detalles Bibliográficos
Autores principales: Omatsu‐Kanbe, Mariko, Yoshioka, Kengo, Fukunaga, Ryo, Sagawa, Hironori, Matsuura, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917088/
https://www.ncbi.nlm.nih.gov/pubmed/29696821
http://dx.doi.org/10.14814/phy2.13688