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New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi

We have created new vectors for clustered regularly interspaced short palindromic repeat (CRISPR) mutagenesis in Candida albicans, Saccharomyces cerevisiae, Candida glabrata, and Naumovozyma castellii. These new vectors permit a comparison of the requirements for CRISPR mutagenesis in each of these...

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Detalles Bibliográficos
Autores principales: Vyas, Valmik K., Bushkin, G. Guy, Bernstein, Douglas A., Getz, Matthew A., Sewastianik, Magdalena, Barrasa, M. Inmaculada, Bartel, David P., Fink, Gerald R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917429/
https://www.ncbi.nlm.nih.gov/pubmed/29695624
http://dx.doi.org/10.1128/mSphere.00154-18