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Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action

Male Sprague‐Dawley rats were given a single intraperitoneal injection of diethylnitrosamine (DEN, 200 mg/kg body weight). Two weeks later the rats were divided into 4 groups; DEN‐C group rats were given no further treatment; DEN‐DES group rats were fed diethylstilbestrol (DES, 0.5 mg/day); DEN‐TMX...

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Detalles Bibliográficos
Autores principales: Kohigashi, Katsuji, Fukuda, Yoshihiro, Imura, Hiroo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917654/
https://www.ncbi.nlm.nih.gov/pubmed/3148604
http://dx.doi.org/10.1111/j.1349-7006.1988.tb01564.x
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author Kohigashi, Katsuji
Fukuda, Yoshihiro
Imura, Hiroo
author_facet Kohigashi, Katsuji
Fukuda, Yoshihiro
Imura, Hiroo
author_sort Kohigashi, Katsuji
collection PubMed
description Male Sprague‐Dawley rats were given a single intraperitoneal injection of diethylnitrosamine (DEN, 200 mg/kg body weight). Two weeks later the rats were divided into 4 groups; DEN‐C group rats were given no further treatment; DEN‐DES group rats were fed diethylstilbestrol (DES, 0.5 mg/day); DEN‐TMX group rats were given tamoxifen (TMX, 1.0 mg/ day) orally; DEN‐DES TMX group rats were fed both DES and TMX for 8 months. Rats of the DEN‐DES group developed grossly visible hepatic tumors. On the other hand, tumor development was significantly inhibited in rats of the DEN‐DES TMX group. Total area of γ‐glutamyl transpeptidase‐positive lesions and the mean area per lesion were significantly larger in rats of the DEN‐DES group than those of the DEN‐C, DEN‐TMX or DEN‐DES TMX group. Estrogen receptor (ER) content of liver cytosol assayed by enzyme immunoassay (EIA) was significantly greater in rats of the DEN‐DES group than in those of the DEN‐C group and smaller in rats of the DEN‐TMX and DEN‐DES TMX group than in the DEN‐C group. On the contrary, ER content of liver nuclei was significantly greater in rats of the DEN‐TMX and DEN‐DES TMX group than in those of the DEN‐C or DEN‐DES group. These results suggest that the promotive action of DES and the inhibitory action of TMX on DES‐promoted hepatic tumorigenesis are, at least in part, mediated by ER in the rat.
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spelling pubmed-59176542018-05-11 Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action Kohigashi, Katsuji Fukuda, Yoshihiro Imura, Hiroo Jpn J Cancer Res Article Male Sprague‐Dawley rats were given a single intraperitoneal injection of diethylnitrosamine (DEN, 200 mg/kg body weight). Two weeks later the rats were divided into 4 groups; DEN‐C group rats were given no further treatment; DEN‐DES group rats were fed diethylstilbestrol (DES, 0.5 mg/day); DEN‐TMX group rats were given tamoxifen (TMX, 1.0 mg/ day) orally; DEN‐DES TMX group rats were fed both DES and TMX for 8 months. Rats of the DEN‐DES group developed grossly visible hepatic tumors. On the other hand, tumor development was significantly inhibited in rats of the DEN‐DES TMX group. Total area of γ‐glutamyl transpeptidase‐positive lesions and the mean area per lesion were significantly larger in rats of the DEN‐DES group than those of the DEN‐C, DEN‐TMX or DEN‐DES TMX group. Estrogen receptor (ER) content of liver cytosol assayed by enzyme immunoassay (EIA) was significantly greater in rats of the DEN‐DES group than in those of the DEN‐C group and smaller in rats of the DEN‐TMX and DEN‐DES TMX group than in the DEN‐C group. On the contrary, ER content of liver nuclei was significantly greater in rats of the DEN‐TMX and DEN‐DES TMX group than in those of the DEN‐C or DEN‐DES group. These results suggest that the promotive action of DES and the inhibitory action of TMX on DES‐promoted hepatic tumorigenesis are, at least in part, mediated by ER in the rat. Blackwell Publishing Ltd 1988-12 /pmc/articles/PMC5917654/ /pubmed/3148604 http://dx.doi.org/10.1111/j.1349-7006.1988.tb01564.x Text en
spellingShingle Article
Kohigashi, Katsuji
Fukuda, Yoshihiro
Imura, Hiroo
Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action
title Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action
title_full Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action
title_fullStr Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action
title_full_unstemmed Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action
title_short Inhibitory Effect of Tamoxifen on Diethylstilbestrol‐promoted Hepatic Tumorigenesis in Male Rats and Its Possible Mechanism of Action
title_sort inhibitory effect of tamoxifen on diethylstilbestrol‐promoted hepatic tumorigenesis in male rats and its possible mechanism of action
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917654/
https://www.ncbi.nlm.nih.gov/pubmed/3148604
http://dx.doi.org/10.1111/j.1349-7006.1988.tb01564.x
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