Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice

In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demeth...

Descripción completa

Detalles Bibliográficos
Autores principales: Araki, Kimi, Miyazaki, Jun‐ichi, Tsurimoto, Toshiki, Inomoto, Takeaki, Iwanaga, Tomohisa, Matsubara, Kenichi, Yamamura, Ken‐ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1989
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917734/
https://www.ncbi.nlm.nih.gov/pubmed/2473052
http://dx.doi.org/10.1111/j.1349-7006.1989.tb02308.x
_version_ 1783317277063512064
author Araki, Kimi
Miyazaki, Jun‐ichi
Tsurimoto, Toshiki
Inomoto, Takeaki
Iwanaga, Tomohisa
Matsubara, Kenichi
Yamamura, Ken‐ichi
author_facet Araki, Kimi
Miyazaki, Jun‐ichi
Tsurimoto, Toshiki
Inomoto, Takeaki
Iwanaga, Tomohisa
Matsubara, Kenichi
Yamamura, Ken‐ichi
author_sort Araki, Kimi
collection PubMed
description In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.
format Online
Article
Text
id pubmed-5917734
institution National Center for Biotechnology Information
language English
publishDate 1989
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59177342018-05-11 Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice Araki, Kimi Miyazaki, Jun‐ichi Tsurimoto, Toshiki Inomoto, Takeaki Iwanaga, Tomohisa Matsubara, Kenichi Yamamura, Ken‐ichi Jpn J Cancer Res Rapid Communication In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice. Blackwell Publishing Ltd 1989-04 /pmc/articles/PMC5917734/ /pubmed/2473052 http://dx.doi.org/10.1111/j.1349-7006.1989.tb02308.x Text en
spellingShingle Rapid Communication
Araki, Kimi
Miyazaki, Jun‐ichi
Tsurimoto, Toshiki
Inomoto, Takeaki
Iwanaga, Tomohisa
Matsubara, Kenichi
Yamamura, Ken‐ichi
Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
title Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
title_full Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
title_fullStr Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
title_full_unstemmed Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
title_short Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice
title_sort demethylation by 5‐azacytidine results in the expression of hepatitis b virus surface antigen in transgenic mice
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917734/
https://www.ncbi.nlm.nih.gov/pubmed/2473052
http://dx.doi.org/10.1111/j.1349-7006.1989.tb02308.x
work_keys_str_mv AT arakikimi demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice
AT miyazakijunichi demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice
AT tsurimototoshiki demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice
AT inomototakeaki demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice
AT iwanagatomohisa demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice
AT matsubarakenichi demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice
AT yamamurakenichi demethylationby5azacytidineresultsintheexpressionofhepatitisbvirussurfaceantigenintransgenicmice

Ejemplares similares