Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells

Human tumor–infiltrating lymphocytes (TILs) derived from pleural or ascitic fluid were incubated with recombinant interleukin 2 and transfected with human tumor necrosis factor (TNF) a gene by the lipofection procedure. The resulting TILs secreted significant amounts of TNF in the culture supernatan...

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Autores principales: Itoh, Yoshinori, Kohgo, Yutaka, Watanabe, Naoki, Kanisawa, Yuji, Sakamaki, Sumio, Takahashi, Minoru, Hirayama, Yasuo, Ono, Hiroyuki, Himeno, Takeshi, Niitsu, Yoshiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1991
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918323/
https://www.ncbi.nlm.nih.gov/pubmed/1752779
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01781.x
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author Itoh, Yoshinori
Kohgo, Yutaka
Watanabe, Naoki
Kanisawa, Yuji
Sakamaki, Sumio
Takahashi, Minoru
Hirayama, Yasuo
Ono, Hiroyuki
Himeno, Takeshi
Niitsu, Yoshiro
author_facet Itoh, Yoshinori
Kohgo, Yutaka
Watanabe, Naoki
Kanisawa, Yuji
Sakamaki, Sumio
Takahashi, Minoru
Hirayama, Yasuo
Ono, Hiroyuki
Himeno, Takeshi
Niitsu, Yoshiro
author_sort Itoh, Yoshinori
collection PubMed
description Human tumor–infiltrating lymphocytes (TILs) derived from pleural or ascitic fluid were incubated with recombinant interleukin 2 and transfected with human tumor necrosis factor (TNF) a gene by the lipofection procedure. The resulting TILs secreted significant amounts of TNF in the culture supernatant and exhibited cytotoxicity against established cell lines, such as K562 and Daudi, and autologous tumor cells. The TNF gene–transfected TILs exhibited an augmented killing of autologous tumor cells.
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spelling pubmed-59183232018-05-11 Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells Itoh, Yoshinori Kohgo, Yutaka Watanabe, Naoki Kanisawa, Yuji Sakamaki, Sumio Takahashi, Minoru Hirayama, Yasuo Ono, Hiroyuki Himeno, Takeshi Niitsu, Yoshiro Jpn J Cancer Res Article Human tumor–infiltrating lymphocytes (TILs) derived from pleural or ascitic fluid were incubated with recombinant interleukin 2 and transfected with human tumor necrosis factor (TNF) a gene by the lipofection procedure. The resulting TILs secreted significant amounts of TNF in the culture supernatant and exhibited cytotoxicity against established cell lines, such as K562 and Daudi, and autologous tumor cells. The TNF gene–transfected TILs exhibited an augmented killing of autologous tumor cells. Blackwell Publishing Ltd 1991-11 /pmc/articles/PMC5918323/ /pubmed/1752779 http://dx.doi.org/10.1111/j.1349-7006.1991.tb01781.x Text en
spellingShingle Article
Itoh, Yoshinori
Kohgo, Yutaka
Watanabe, Naoki
Kanisawa, Yuji
Sakamaki, Sumio
Takahashi, Minoru
Hirayama, Yasuo
Ono, Hiroyuki
Himeno, Takeshi
Niitsu, Yoshiro
Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
title Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
title_full Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
title_fullStr Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
title_full_unstemmed Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
title_short Human Tumor–infiltrating Lymphocytes Transfected with Tumor Necrosis Factor Gene Could Augment Cytotoxicity to Autologous Tumor Cells
title_sort human tumor–infiltrating lymphocytes transfected with tumor necrosis factor gene could augment cytotoxicity to autologous tumor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918323/
https://www.ncbi.nlm.nih.gov/pubmed/1752779
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01781.x
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