Transforming Activity of a Synthetic c‐Ha‐ras Gene Containing O(6)‐Methylguanine in Codon 12

A mutagenic DNA‐adduct, O(6)‐methylguanine, was introduced into codon 12 of the synthetic c‐Ha‐ras gene by cassette mutagenesis. Transfection of this modified ras gene into normal NIH3T3 cells by the calcium phosphate procedure resulted in significant induction of focus formation. The ras gene inser...

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Detalles Bibliográficos
Autores principales: Kamiya, Hiroyuki, Miura, Kazunobu, Ohtomo, Noriko, Nishimura, Susumu, Ohtsuka, Eiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1991
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918606/
https://www.ncbi.nlm.nih.gov/pubmed/1938604
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01934.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918606/
https://www.ncbi.nlm.nih.gov/pubmed/1938604
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01934.x

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