Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci

Loss of heterozygosity (LOH) at APC and MCC gene loci (both mapped to 5q21) was investigated in 24 surgical specimens of primary gastric carcinomas using the polymerase chain reaction after tumor cell enrichment by cell sorting based on differences in DNA content. LOH at APC and/or MCC was detected...

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Autores principales: Tamura, Gen, Maesawa, Chihaya, Suzuki, Yasushi, Ogasawara, Satoshi, Terashima, Masanori, Saito, Kazuyoshi, Satodate, Ryoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1993
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919060/
https://www.ncbi.nlm.nih.gov/pubmed/8226275
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02794.x
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author Tamura, Gen
Maesawa, Chihaya
Suzuki, Yasushi
Ogasawara, Satoshi
Terashima, Masanori
Saito, Kazuyoshi
Satodate, Ryoichi
author_facet Tamura, Gen
Maesawa, Chihaya
Suzuki, Yasushi
Ogasawara, Satoshi
Terashima, Masanori
Saito, Kazuyoshi
Satodate, Ryoichi
author_sort Tamura, Gen
collection PubMed
description Loss of heterozygosity (LOH) at APC and MCC gene loci (both mapped to 5q21) was investigated in 24 surgical specimens of primary gastric carcinomas using the polymerase chain reaction after tumor cell enrichment by cell sorting based on differences in DNA content. LOH at APC and/or MCC was detected in 87% (13/15) of the cases; at the APC in 86% (12/14) and at the MCC locus in 100% (7/7). LOH at the APC locus was always accompanied by LOH at the MCC locus. LOH at the APC and/or MCC was found in both differentiated and undifferentiated types in both early and advanced stages of gastric carcinoma. Thus, LOH at APC and/or MCC is considered to be one of the most prevalent genetic alterations in human gastric carcinoma and occurs at an early stage of the carcinogenesis.
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spelling pubmed-59190602018-05-11 Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci Tamura, Gen Maesawa, Chihaya Suzuki, Yasushi Ogasawara, Satoshi Terashima, Masanori Saito, Kazuyoshi Satodate, Ryoichi Jpn J Cancer Res Rapid Communication Loss of heterozygosity (LOH) at APC and MCC gene loci (both mapped to 5q21) was investigated in 24 surgical specimens of primary gastric carcinomas using the polymerase chain reaction after tumor cell enrichment by cell sorting based on differences in DNA content. LOH at APC and/or MCC was detected in 87% (13/15) of the cases; at the APC in 86% (12/14) and at the MCC locus in 100% (7/7). LOH at the APC locus was always accompanied by LOH at the MCC locus. LOH at the APC and/or MCC was found in both differentiated and undifferentiated types in both early and advanced stages of gastric carcinoma. Thus, LOH at APC and/or MCC is considered to be one of the most prevalent genetic alterations in human gastric carcinoma and occurs at an early stage of the carcinogenesis. Blackwell Publishing Ltd 1993-10 /pmc/articles/PMC5919060/ /pubmed/8226275 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02794.x Text en
spellingShingle Rapid Communication
Tamura, Gen
Maesawa, Chihaya
Suzuki, Yasushi
Ogasawara, Satoshi
Terashima, Masanori
Saito, Kazuyoshi
Satodate, Ryoichi
Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci
title Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci
title_full Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci
title_fullStr Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci
title_full_unstemmed Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci
title_short Primary Gastric Carcinoma Cells Frequently Lose Heterozygosity at the APC and MCC Genetic Loci
title_sort primary gastric carcinoma cells frequently lose heterozygosity at the apc and mcc genetic loci
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919060/
https://www.ncbi.nlm.nih.gov/pubmed/8226275
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02794.x
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